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In Search of Putative FOXP3+ Cell Surface Markers By Expert Author: Stephen Jones
View Summary \| Submitted: 2008-07-11 \| Word Count: 203 words \| Views: 53 view(s)

Despite intense interest and scrutiny focused on FOXP3 as a key protein and master transcription factor, isolating and enriching for viable FOXP3 positive cells remains a challenge. Although cell separation/staining via CD4+CD25+ selection is commonly used, this technique has limited applications. Thus, surface markers specific for FOXP3 positive cells would be invaluable research tools as they would:

• Facilitate isolation and purification of viable
Treg cells
• Distinguish naturally occurring
CD4+CD25+cells from both naive and
recently activated CD4+CD25-
nonregulatory T cells
• Allow therapeutic manipulation of
Treg cells

As the search for putative FOXP3+ markers continue, Neuropilin-1, GPR83, and FR4 have emerged as potential candidates. IMGENEX is excited to offer a panel of flow cytometric characterized antibodies against:

• Neuropilin-1 (clone 211H6.01)
• GPR83 (polyclonal)
• Folate Receptor 4 (clones 12A5 and TH6)

Flow cytometric analysis of intracellular FOXP3 (IMG-5802D) and cell surface FR4 with clone 12A5 (IMG-6217C) (left) and clone TH6 (IMG-6218C) (right) at 0.06 ug/10^6 mouse splenocytes.

Flow cytometric analysis of Neuropilin-1 in CD4+CD25+ human PBMCs using A) an isotype control and B) DDX0440 at 0.5 ug/10^6 cells. These antibodies are available in multiple sizes and conjugates.

About the Author/Author Bio

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of IMGENEX Corporation, San Diego, USA. Find out more information about FOXP3+ Cell Surface Markers.

Article Source: http://www.articlesphere.com/Article/In-Search-of-Putative-FOXP3--Cell-Surface-Markers/154444

Article Tags: alternative medicine, foxp3, cell surface markers

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